ypfD [2019-09-18 09:48:00]

similar to ribosomal protein S1

locus: BSU_22880

pI: 4.57

mw: 42.24 kDa

protein length: 382 aa

gene length: 1149 bp

function: unknown

product: unknown

essential: no

synonyms: jofD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.5|Ribosomal protein/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 2,394,664 2,395,812

The protein

Protein family

• bacterial [SW|ribosomal protein] bS1 family (single member, according to UniProt)

[SW|Domains]

• 4 reiterated [SW|S1 domain]s (aa 13 ...84, aa 98 ... 167, aa 185 ... 256, aa 269 ... 342) (according to the Interpro database)

Modification

• phosphorylation on Ser-243 [Pubmed|17218307]

Structure

• [PDB|5X8R] (chloroplast, small ribosomal subunit, 37% identity to aa 14 .. 266) [pubmed|28582576]
• [PDB|1SRO] (E. coli S1 RNA-binding domain, 37% identity to aa 185 ... 343) [pubmed|9008164]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]
  description: [pubmed|22383849]

• genes: [gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

• genes: [gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

• genes: [gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]
  description: [pubmed|22383849]

• genes: [gene|C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE|motI]-[gene|3459BA0CDAABC288ADF97B111F78C7EAC184840D|ypfB]-[gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

• genes: [gene|C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE|motI]-[gene|3459BA0CDAABC288ADF97B111F78C7EAC184840D|ypfB]-[gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE22880 ([gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATAACCTCCTTGG, downstream forward: _UP4_TAATTGGTGATAAACGTGAC
• BKK22880 ([gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATAACCTCCTTGG, downstream forward: _UP4_TAATTGGTGATAAACGTGAC

References

• 17218307,20525796,23002217,15378759,28516784,28582576,9008164